Background- We aimed to find out the spectrum of bacterial infections in ascitic fluid among patients of decompensated chronic liver disease in a tertiary care hospital. Methodology- This study was conducted as a facility based cross sectional observational study on cases of decompensated Chronic Liver disease having ascites, seeking care at People’s Hospital Bhopal during the study period of 1st November 2022 to 30th April 2024. History and examination was done and findings were recorded. Diagnostic paracentesis was done and ascitic fluid subjected to examination. Results-Ascitic fluid biochemistry revealed low total protein levels in 47.2% cases. SAAG was less than 1.1 in 18.5% cases and more than 1.1 in 71.5% cases of decompensated Chronic Liver Disease.Gram staining revealed pus cells in 10 (8.3%) cases and Ascitic fluid culture was positive in 1.8% cases. ADA levels were raised in 1 (0.9% cases). Spontaneous bacterial peritonitis was present in 7.4% of all the sample analysed. Conclusions-Ascitic fluid analysis helps in evaluation of patients with Chronic Liver Disease and helps in adequate treatment and predicting the outcome of patients with decompensated chronic liver disease. We found pus cells in 10 patients out of total 108 patients on Gram stain, however, organisms were isolated in only 2 patients i.e. Enterococcus species and Pseudomonas Aeruginosa in one each.