Background: Protein energy malnutrition affects chronic Hemodialysis patients and is associated with significant morbidity and mortality. Malnutrition is related to inflammation, diminished dietary intake, adequacy of dialysis, socio economic factors. Various biochemical parameters like urea, creatinine, calcium, phosphorous, albumin, pre albumin, cholesterol, transferrin is measured. Anthropometric measurements like height, body weight, triceps skin fold thickness, mid arm circumference, mid arm muscle circumference is measured. Aim of this study is to assess malnutrition in chronic Hemodialysis patients through various Biochemical parameters and correlate them with anthropometric measurements. Materials & methods: All the participants or their attenders were informed about the study and informed consent was obtained from them. A brief history about their personal details such as life style, physical activity, food habits, their socioeconomic status, history of any illness was obtained. General examination and systemic examination were done. The study population N = 85 in the age group of > 25 years in both sexes under chronic Hemodialysis for more than three months. After getting the informed consent from the patient and patient attender, 4ml of blood was collected for biochemical investigations. Results: In this study, there was malnutrition among the Hemodialysis patients. P value was found to be significant for Cholesterol, Albumin, Prealbumin, Transferrin by One way ANOVA .Pearson Correlation between SGA score and Cholesterol, Albumin, Phosphorus, Calcium, Prealbumin, Transferrin showed statistically significant negative linear relationship .In our study Significant correlation is not seen for the Biochemical parameters and Anthropometric measurements like Body mass index, Mid arm muscle circumference. Conclusion: Malnutrition is one of the main causes of mortality and morbidity among Chronic Hemodialysis patients. So, Assessment of malnutrition should be done even before the initiation of Hemodialysis which will help in the favorable outcome of the Hemodialysis patients. Several parameters are available for the assessment of malnutrition, of which Biochemical parameters play a major role.