Background:A healthy term infant is the result of three critical components: a well-nourished mother, normal genetic factors, and effective placental implantation and development. The placenta, while often overlooked, is a vital organ. Its proper functioning is essential for the adequate growth and development of the fetus. Hence; the present study was conducted for assessing the correlation between placental thickness and amniotic fluid index in third trimester of uncomplicated pregnancy in Indian females. Materials & methods: 200 patients who were recruited for the study were categorized into four distinct groups based on their gestational age: 26 to 30 weeks, 31 to 34 weeks, 35 to 38 weeks and 39 to 42 weeks. The gestational age of participants was verified through prior ultrasonography reports from the first trimester. Comprehensive ultrasound examinations were conducted, during which the placenta was identified as a hyper-echoic region, delineated from the fetus by a hypo-echoic area filled with amniotic fluid. The thickness of the placenta was measured, and the Amniotic Fluid Index (AFI) was calculated by summing the vertical measurements of the deepest fluid pockets across the four quadrants of the uterus. Results:A total of 200 subjects were evaluated. Mean age of the subjects was 29.3 years. 41.5 percent of the subjects belonged to the gestational age group of 35 to 38 years. Among subjects with gestational age of 26 to 30 weeks, 31 to 34 weeks, 35 to 38 weeks and 39 to 42 weeks, mean placental thickness was 3.62 cm, 4.08 cm, 4.13 cm and 3.89 cm respectively. Among subjects with gestational age of 26 to 30 weeks, 31 to 34 weeks, 35 to 38 weeks and 39 to 42 weeks, mean AFI was 146.3 mm, 138.2 mm, 121.3 mm and 96.7 mm respectively. Significant negative correlation of AFI with gestational age was seen. However; no significant correlation of placental thickness with gestational age was seen. No-significant correlation of placental thickness with AFI was seen. Conclusion:Normal placental function and structure are required for normal growth and development of the fetus. Placental thickness is the simplest measurement of placental size and can be measured at any center equipped with ultrasound machine. No-significant correlation of placental thickness with gestational age was seen. AFI significantly correlated with gestational age.