Platelets are vital for maintaining hemostasis and vascular integrity, especially in neonatal patients who are in a critical stage of development. It is important to understand the complex mechanisms of platelet production, function, and regulation in neonates to ensure the best health outcomes for this population. In our study, we focused on neonates born at the PES Institute of Medical Sciences and Research, as well as those referred for specialized care. We measured platelet counts and related indices for 140 neonates using automated analyzers, and we collected a detailed clinical history for each newborn. Neonatal blood was drawn into single-use collection tubes with K2 EDTA. The automated haematology analyser, SYSMEX XS-1000i, provided the platelet indices. In our study, the frequency of full-term neonates and preterm neonates were 115 and 25 respectively. The current study found that 23.6% of neonates had decreased PC, 1.4% had an increased count, and 75% had normal values. In our study,we found that there were no statistically significant differences between the MPV, PDW, and PCT values of full-term and preterm infants. Regularly monitoring platelet levels is essential in neonatal care, helping to quickly identify potential issues and prevent serious complications. This proactive approach ensures that vulnerable infants receive the attention they need, allowing healthcare providers to take prompt action and giving newborns a better chance at a healthy start.