Background: Trauma significantly contributes to global morbidity and mortality. Serum lactate variation reflects the balance between oxygen demand and supply, making it a valuable prognostic tool in trauma resuscitation. This study examines serum lactate levels in predicting outcomes in trauma patients. Methods: Trauma patients with an Injury Severity Score (ISS) >15 were included. Serum lactate levels were measured at admission and 2 hours post-resuscitation using the ATLS protocol. Patient outcomes were documented at discharge. Statistical Analysis: SPSS Version 21.0 and Microsoft Excel 2010 were used for analysis and graphical representation. ROC (Receiver Operating Characteristic) curve analysis assessed the predictive accuracy of ISS and lactate levels for mortality. Results: The mean patient age was 39.9 years, with road traffic accidents accounting for 72.3% of injuries. The overall mortality rate was 26.2%, with 10.8% occurring within 48 hours. Median lactate levels declined from 1.10 mmol/L at admission to 0.80 mmol/L after 2 hours (p<0.01). Higher ISS and lactate levels correlated with mortality. ROC analysis showed ISS (AU-ROC 0.957) and lactate levels at 2 hours (AU-ROC 0.94) as strong predictors of mortality. ISS >35 demonstrated 100% sensitivity and 83.3% specificity, while lactate >1.1 mmol/L at 2 hours had 100% sensitivity and 72.9% specificity. Conclusion: Elevated ISS and lactate levels at admission and 2 hours predict higher mortality, especially within 48 hours. ROC analysis highlights the utility of ISS and lactate for early risk stratification in trauma patients.