Background: Burkholderia cepacia complex (Bcc) is a group of gram-negative, non-fermentative bacteria primarily recognized as opportunistic pathogens, particularly in immunocompromised patients and those with cystic fibrosis. While commonly associated with respiratory infections, B. cepacia has increasingly been identified in diverse anatomical sites, including the female reproductive tract, albeit rarely. Case Report: This report describes an unusual case of B. cepacia isolated from the endometrial fluid of a 58-year-old postmenopausal woman presenting with abnormal vaginal bleeding. Initial assessments, including imaging and laboratory tests, suggested possible pyometra or cervical stenosis, prompting diagnostic hysteroscopy and microbiological analysis. The organism was cultured on selective media and characterized biochemically, confirming its identity and demonstrating resistance to multiple antibiotics, while remaining susceptible to meropenem and trimethoprim-sulfamethoxazole. Discussion: This case underscores the necessity for vigilant microbiological investigation when atypical organisms are suspected, particularly in the absence of overt immunocompromising conditions. Given the pathogen’s potential to cause significant morbidity, timely identification and appropriate antibiotic therapy are crucial to managing such infections effectively. Conclusion: This report emphasizes the importance of recognizing B. cepacia as a possible pathogen in gynecological contexts and the need for heightened clinical awareness.