Introduction: Eagle's syndrome, characterized by elongated styloid processes or calcified stylohyoid ligaments, is an intriguing condition frequently associated with symptoms such as throat torment, dysphagia, otalgia, and facial torment.Objectives: The main objective of the study is to find the radio-anatomical variations of temporal bone in diagnosed patients of eagle's syndrome. Material and methods: This retrospective study was conducted and data was collected from 20 diagnosed patients of eagle's syndrome. A review of medical records and radiological images was conducted for all eligible patients diagnosed with Eagle's syndrome who met the inclusion criteria. Relevant clinical information, including patient demographics, presenting symptoms, and medical history, was extracted from electronic medical records. CT and MRI images of the temporal bone were retrieved from the hospital's data and reviewed by experienced radiologists. Results: Data were collected from 20 patients diagnosed with Eagle's syndrome. There were 12 male and 08 female patients. Mean age of male patients were 47.29 ± 7.5 years and female were 42.38 ± 9.1 years. The most common variation observed was an elongated styloid process, found in 75% of patients. Calcification of the stylohyoid ligament was also frequent, with 50% of patients exhibiting this variation. Among the calcified cases, complete calcification was more prevalent than partial calcification. Pneumatization and aberrant bony spurs were observed in 25% and 15% of patients, respectively. Statistical analysis revealed significant differences between male and female measurements for both the right (p = 0.0002) and left (p = 0.0001) sides. However, the difference in measurements between the right and left sides within each gender group was not statistically significant (p = 0.1629 for males, p = 0.4618 for females).Conclusion: It is concluded that Eagle's syndrome presents with notable variations in the temporal bone, including asymmetry in styloid process measurements and diverse patterns of calcification. These findings highlight the importance of radiological assessments, considering gender-specific anatomical differences and bilateral involvement.