Background: Due to the increasing scenario of UTI and the prevalence of antimicrobial resistance uropathogens, the study focuses on the prevailing gram-negative bacteria (GNB)causing UTI and its associated co-factors. Methodology: It is a retrospective cross-sectional study for a period of 3 years from January 2021 to December 2023 from patients suspected of UTI. Urine samples were collected and processed microbiologically according to SOP. Antibiotic susceptibility testing was performed by CLSI guidelines. Result: E. coli (60.8%, n=4333) and Klebsiella sp. (23.6%, n=4333) are the predominant GNB causing UTI. Female patients (59.8%, n=4333) in comparison to male patients were more affected. The age group 51 to 70 years (35%, n=4333) were more affected. The highest sensitivity was observed from fosfomycin (87.9%, n=4333), followed by amikacin (81.3%, n=4333). Klebsiella sp. has a higher rate of resistance to antibiotics. Among the E. coli and Klebsiella sp., 24.5% (n=3662) are susceptible strains, whereas 63.1% (n=3662) and 12.2% (n=3662) of the strains are multidrug-resistant (MDR) and extensively drug-resistant (XDR), respectively. Conclusion: In this region, the prevalence of UTIs has increased alarmingly in the last two to three years. The aetiological agent may vary depending on age, gender, and season. The source of isolation (ICU, OPD, or ward) may have an impact on the antimicrobial susceptibility pattern of the aetiological agent within a region. Strains from the ICU have more resistance frequencies. To reduce the prevalence of MDR and XDR, the study recommends a mandatory antibiogram profile before starting UTI treatment.