Background: The calcitonin hormone is vital in regulating phosphorus and calcium homeostasis. Also, in systemic inflammation, such as bacterial infections, procalcitonin is produced in various tissues. The procalcitonin level rises 2-4 hours post-stimulation and reaches a maximum in 6-2 hours. However, the literature data is mainly dependent on sepsis-induced bacterial infection. Aim: The present study evaluated the procalcitonin test's ability to discriminate various bacterial (non-sepsis) etiologies in a large population of Indian subjects. Methods: The present study was assessed utilizing routine laboratory and clinical data gathered from the Department of Microbiology of the Institute within the defined study period. The data gathered were used to assess the significance of serum biomarker C reactive protein, procalcitonin test, and total leucocyte count for early detection of bacterial infection. Results: The study results showed a higher prevalence of increased levels of procalcitonin in cases of gram-negative bacterial infections, particularly in infections of Klebsiella pneumoniae and Escherichia coli in comparison to the population with gram-positive bacterial infections. Conclusions: The present study concludes that there is an increase in PCT levels even in subjects with non-septic bacterial infections as an increase in CRP and TLC which are taken as the standard of serum biomarkers in any bacterial infection. It is also seen that the increase in these biomarkers in gram-negative and gram-positive infected subjects is similar irrespective of gender. Also, raised PCT is higher in gram-negative infections compared to gram-positive infections.