Objective: Hookworm infestation contribute significantly to the disease burden in developing nations. The purpose of this study is to know about hookworm infections- Ancylostoma duodenale and Necator americanus, in patients reporting at tertiary care hospital in Raipur. Methods: This was a retrospective observational study. A total of 659 stool samples received from patients with various gastro-intestinal disturbances were microscopically screened for the presence of hookworm eggs. Hookworm eggs in freshly passed unfixed stool samples were cultured to the infective third stage filariform larvae at room temperature, by the Harada Mori filter paper strip method. The cultured filariform larvae were examined under the microscope; both iodine stained and unstained larvae were examined and identified according to the criteria of WHO. Results: Out of 659 stool samples that were microscopically screened for the presence of hookworm eggs, 50 samples were found positive for different parasitic infections of which 20 cases of hookworm infection were recorded giving us an overall infection rate of 40%. Of the 10 hookworm positive coproculture N.americanus filariform larvae were recovered from a total of 9 [90%] while A.duodenale larvae were recovered from a total of 3[30%] patients. Only 2[20%] of the coprocultures had double species infections as evidenced by the recovery of the filariform larvae of both species. However in all mixed infection higher number of N.americanus larvae were recovered compared to A.duodenale, the former ranging from 62-75% and the latter ranging from 25-60%. Conclusions: Hookworm infection ranks quite high among the patients with different gastro intestinal disturbances. This is alarming as because hookworm has been associated with impaired cognitive functions and decreased future economic productivity