Aim: This study aimed to evaluate the morphometric characteristics and the incidence of anatomical variations in the gallbladder, cystic duct, and common bile duct (CBD) using ultrasonography, and to highlight their clinical and surgical implications. Material and Methods: A cross-sectional observational study was conducted on 90 participants at the Departments of Anatomy and Radiodiagnosis, Government Medical College Anantnag, Jammu & Kashmir. Participants underwent ultrasonographic evaluation of the gallbladder, cystic duct, and CBD for morphometric measurements and identification of anatomical variations. Inclusion criteria were individuals aged ≥18 years with no prior hepatobiliary surgery, while exclusion criteria included malignancies, severe obesity, and unwillingness to consent. Data were analyzed using descriptive statistics, with significance assessed at p<0.05. Results: The mean gallbladder length, width, and volume were 7.50 ± 1.20 cm, 3.20 ± 0.80 cm, and 28.50 ± 5.10 mL, respectively, with a significant variation (p<0.05). Wall thickness averaged 2.50 ± 0.30 mm. Normal gallbladder morphology was observed in 88.89% of participants, while 11.11% exhibited variations, including ectopic positioning (4.44%), double gallbladder (3.33%), and septate gallbladder (2.22%). Cystic duct variations were noted in 8.89%, with spiral valves (4.44%) being the most common. The CBD was normal in 94.44%, while variations like accessory ducts (2.22%) and choledochal cysts (1.11%) were identified. Significant morphometric differences in CBD diameter (0.70 ± 0.15 cm) and length (8.10 ± 1.30 cm) were also observed (p<0.05). Conclusion: This study revealed significant morphometric variability and notable anatomical variations in the gallbladder, cystic duct, and CBD. Variations such as ectopic gallbladder positioning, spiral valves in the cystic duct, and accessory bile ducts emphasize the importance of detailed ultrasonographic assessment. These findings highlight the need for preoperative evaluation to mitigate surgical risks and improve clinical outcomes.