Background: Despite advances, early and accurate tuberculosis (TB) diagnosis remains challenging, particularly in high-burden regions like India.Apart from the conventional methods, Mycobacterium Growth Indicator Tube (MGIT 960), a non-radiometric liquid culture, has rapid turnover and a good recovery rate. The use of MGIT is still limited due to high burden on resources. This study is designed to assess the performance of MGIT in comparison toother tests for the detection of TB. Methods: A prospective observational study was conducted on 778 pulmonary samples collected between January 2023 and June 2024 at a tertiary care center in North India, using microscopy, solid culture (Lowenstein-Jensen medium), liquid culture (MGIT 960), and the CBNAAT. The samples were processed using standard procedure and manufacturer’s protocol. Data was analyzed using SPSS 23.0. The sensitivity, specificity, predictive value, and average turnaround time were calculated. Results: Out of 778 samples, 223 (28.7%), isolates were detected MTB by MGIT, followed by 216(27.8%) isolates from CBNAAT along with 3 errors, 190 (24.4%) by Solid culture along with 7 contaminated culture and 85 (10.9%) isolates by microscopy. The MTB is detected significantly faster in MGIT cultures than in LJ cultures. The agreement between MGIT 960, solid culture and CBNAAT was high (Kappa= 0.891, Kappa= 0.978 respectively). Conclusion: MGIT is an automated method and provides quicker results than conventional methods like microscopy and solid culture. The results are comparable to other conventional tests includingCBNAAT.The findings underscore the importance of advanced diagnostic tools in improving TB management and reducing detection delays, particularly in regions with limited resources.