Background:Platelet-Rich Plasma (PRP) therapy has emerged as a promising regenerative technique in musculoskeletal medicine. PRP is an autologous preparation enriched with platelets, growth factors, and bioactive molecules that promote tissue regeneration and accelerate healing. Despite its widespread application, the precise mechanisms and effectiveness of PRP in treating musculoskeletal injuries remain under investigation. Materials and Methods:A prospective study was conducted on 50 patients aged 20–50 years with musculoskeletal injuries, including ligament tears, tendon injuries, and muscle strains. PRP was prepared by centrifuging the patient's blood to achieve a platelet concentration of approximately 5x baseline. Patients were administered PRP injections at the injury site under ultrasound guidance. Outcomes were assessed using visual analogy scale (VAS) scores for pain and functional improvement using a validated Musculoskeletal Function Scale (MFS) at baseline, 4 weeks, and 12 weeks post-treatment. Results:Patients demonstrated significant improvement in pain and functional outcomes. The mean VAS score decreased from 7.5 ± 1.2 at baseline to 3.2 ± 0.8 at 4 weeks and 1.8 ± 0.5 at 12 weeks (p < 0.05). MFS scores improved from 40.3 ± 5.1 to 75.6 ± 4.7 at 12 weeks. No significant adverse effects were reported. PRP therapy was particularly effective in tendon injuries compared to ligament and muscle injuries. Conclusion:PRP therapy effectively promotes tissue regeneration and accelerates healing in musculoskeletal injuries, with significant improvements in pain and function. The treatment is safe, minimally invasive, and holds promise for broader clinical application. However, further large-scale studies are necessary to standardize protocols and confirm long-term benefits.