Background:Triple-negative breast cancer (TNBC) represents a highly aggressive form of breast carcinoma. This research aimed to examine the expression of p53 in TNBC cases and analyze its correlation with various clinical and pathological parameters, including age, tumor size, grade, lymph node involvement, and others. Materials and Methods: The study included 65 cases of histologically and immunohistochemically confirmed TNBC. A detailed clinical data collection was performed for each patient, and the data was recorded systematically using a pre-designed proforma to ensure consistency and accuracy in documenting patient demographics, clinical presentation, and relevant histopathological findings. After histopathological analysis, all the cases were subjected to immunohistochemical (IHC) staining to evaluate the expression of p53. Results:65 female TNBC patients, aged 30-80 years (most commonly 41-50), were analyzed. The left breast was affected in 58.8%, and the upper outer quadrant in 64.6%. Tumor sizes ranged from 1 to 8 cm, with 79.9% between 2-5 cm. Most cases (93.4%) were IDC-NOS, with 60.1% grade III and 83.8% showing lymphovascular invasion (LVI), linked to higher tumor grade (p<0.05). Lymph node involvement was present in 95.1%, more prevalent in premenopausal women (p<0.05). p53 expression was observed in 62.3%, correlating with LVI (p<0.05). No associations were found with tumor size or perineural invasion. Conclusion:This study demonstrated that overexpression of p53 was predominantly associated with high-grade tumors and an increased likelihood of metastasis. Therefore, p53 may serve as a valuable prognostic marker and could be considered a potential target for therapy.