Background: Chronic Suppurative Otitis Media (CSOM) is considered as one of the important health issue which has imposed significant challenges to otorhinolaryngologists . Globally about 65 to 330 million people suffer from CSOM. Keeping the scenario in mind the present study was conducted to determine the prevalence and related risk factors, microbiological profile and its sensitivity pattern of CSOM. Materials and Methods: This cross-sectional study included a total of 122 ear swab specimens of clinically diagnosed individuals with active CSOM. Swabs were cultured in appropriate media, biochemical reactions were performed for the microbial organism identification according to a standard protocol.The Kirby-Bauer disc diffusion method was used where the diameter of the inhibition zone was interpreted according to Clinical Laboratory Standards Institute(CLSI) guidelines. Results: Microbial growth was seen in Seventy-five (75, 61.47%) samples, but forty-seven (47,38.5%) samples had no growth. Among the samples with growth, 72 (96%) were monomicrobial, while 3(4%) were polymicrobial. The most common bacteria isolated was Pseudomonas aeruginosa (23.77%) followed by Staphylococcus aureus (20.49%) and Klebsiella spp.(3.27%). The most sensitive antibiotics against P. aeruginosa was Meropenem. S aureus showed highest sensitivity towards vancomycin (100%). Females were more commonly affected than males. Culture positive CSOM was more common in patient with Diabetes mellitus, people from rural area, in those having frequent respiratory tract infections, throat infections during the episode ear discharge, and vertigo. Conclusion: Upper respiratory tract infection patients and Diabetics are highly prone for developing CSOM with high anti-microbial resistance of P.aeruginosa.