Background: Pseudocysts are either directly or indirectly related to the pancreatic duct system through the pancreatic parenchyma. The usefulness of serum testing is limited. Although they may be within reference ranges, amylase and lipase levels are frequently high. Certain laboratory tests may offer hints about the underlying cause of pancreatitis. The current study's objective was to examine the biochemical makeup of pseudocysts that developed during acute and chronic pancreatitis, with a particular emphasis on proteolytic activity, and to link the biochemical results with each patient's clinical trajectory. Methodology: This study was done in Government Stanley Medical College for a period of 12 months as a ccomparative cross-sectional study in 29 patients. Pseudocyst Pancreas of patients with age 18 to 60 admitted in Surgical Gastroenterology department was included in this study.5 ml of venous blood was collected in plain tube under aseptic precautions. Sample allowed to clot by waiting for 20 minutes. After that Blood and cystic fluid samples was centrifuged at 2500rpm for 15 minutes. Serum was separated and subjected to biochemical analysis and cystic fluid supernatant are stored separately at -20oC for analysis. Results: The mean Serum Amylase among the subjects was 576.07(± 818.14. The mean Serum Lipase among the subjects was 444.17 (± 440.78). The mean Total bilurubin among the subjects was 1.28 (± 0.78). The mean Direct bilirubin among the subjects was 0.63 (± 0.53). The mean Serum protein among the subjects was 6.34 (± 0.77). The mean Serum albumin among the subjects was 3 (± 0.58. The mean Serum Urea among the subjects was 34.93 (± 27.88). The mean Serum creatinine among the subjects was 0.86 (± 0.39). Conclusion: The idea behind the present study began to reduce morbidity and mortality in cases with vascular complications both in acute and chronic pancreatitis with pseudo cyst. Patients diagnosed as pseudocyst by ultrasound or CT scan, should be investigated along with routine parameters , which will give us a clue ,which patients will go for vascular complications and who will recover normally with conservative management, and frequently watch observe and monitor the susceptible patients going for complications in a tertiary care hospital, so that mortality and morbidity can be prevented.