Background: Cervical cancer is a preventable disease due to its long invasive state and availabilities of screening programs, the aim was to diagnose the cervical precancerous lesion by cytology, colposcopy and co-polarized fluorescence spectroscopy with aid of in vivo hand held probe and to compare the efficacy of these modalities in detection of cervical precancerous lesion. Material and Method: It was a prospective non- randomised study conducted in 34 patients who came in the department of Obstetrics and gynaecology, LLR Hospital Kanpur between December 2016 to August 2018. All patients were subjected to conventional PAP smear followed by evaluation by using hand held probe based on co-polarised fluorescence. After that these Patients were subjected to colposcopy guided biopsy and the sample was sent to pathology department of G.S.V.M Medical College for histopathology. The findings were then collaborated, considering histopathology as the gold standard. Results: The sensitivity of hand held probe in detecting precancerous lesions using co-polarized light was 96.67% and specificity was 93.35%. Sensitivity of PAP smear was 84.2% and specificity was 73.3% and colposcopy sensitivity is 73.7% and specificity was 86.7%. Conclusion: The hand held probe based on co-polarized fluorescence spectroscopy is an excellent screening modality that helps in detection of precancerous lesions of the cervix. It has added advantage that it is minimally invasive, can be used in rural set up by paramedics after little training and can be used for mass population screening.