Background: Recently, there has been a lot of focus on the connection between serum paraoxonase enzyme activity and dyslipidemia in obese people, highlighting the interdependence of lipid profiles and enzymatic performance in the context of obesity. The present study was conducted to assess serum paraoxonase enzyme activity in obese individuals. Materials & Methods: 135 obese subjects of both genders were enrolled. Serum paraoxonase activity was measured using a spectrophotometric enzymatic method. Results: Out of 135 patients, 70 were males and 65 were females. The mean BMI was 32.6 kg/m² in males and 31.4 kg/m² in females. The mean age was 44.3 years in males and 45.1 years in females. The mean serum paraoxonase activity was 64.9 U/L in males and 62.4 U/L in females. The difference was significant (P< 0.05). There were 32% male and 16% female drinkers and 68% male and 84% female non- drinkers. There were 33% male and 24% female smokers and 67% male and 76% female non- smokers.There Physical activity was sedentary in 52% male and 68% females and active 48% male and 32% females. The difference was significant (P< 0.05). Conclusion: It was discovered that obese people as compared to non- obese have decreased serum paraoxonase enzyme activity.