Introduction: Healthcare professionals (HCPs) are more likely to experience irregular sleep patterns, which leads them to disregard their own and others' health and well-being. Vigilance, attentiveness, and alertness are all impacted by sleep deprivation (SD). Sleep deprivation has been found to primarily impact the prefrontal cortex of the brain. Furthermore, it slows your reflexes, which hinders performance. Aim: To compare the sleep quality of emergency health care workers and in-patient department health workers in a tertiary care hospital. Material and method: After receiving approval from the institute's ethical council, the current comparative study was carried out at Government Medical College in Kathua, Jammu & Kashmir, over a six-month period and collected and studied the data of a total 200 healthcare workers working in emergency unit and in patient departments. The collected data was analysed with the help of social science software (SPSS), version 22.0. Results: It was observed that the average total sleep quality score for healthcare workers in emergency rooms was 6.63±3.12, whereas the average score for healthcare workers caring for patients was 7.25±4.37. Conclusion: It is concluded that healthcare personnel working in emergency and inpatient departments had significantly different sleep quality.