Background: Benign prostatic hyperplasia (BPH) is one of the most common causes of Lower Urinary Tract Symptoms (LUTS). In this study we attempt to find out the bacterial and fungal aetiological agents of urinary tract infection among Benign Prostatic Hyperplasia patients and their antimicrobial susceptibility pattern. Materials and Methods: Present study was Hospital based Cross sectional study, conducted in adult patients diagnosed as having BPH admitted for Trans Urethral Resection of Prostate (TURP). Using a pretested questionnaire, brief history and investigation details were collected. Urine samples of BPH patients send to MCH Microbiology were processed, organism identified, antibiotic susceptibility testing done. Results: Out of the 203 BPH patients’ urine samples, 113 (55.66%) were sterile and 90 (44.33%) yielded isolates. Monomicrobial organisms constitute 42.85% (n=87) and polymicrobial 1.5% (3). Among the 90 isolates, 87 (96.6%) were monomicrobial, of which 83 were bacteriae and 4 were fungi. In 3 ( 3.33%) polymicrobial isolates , two isolates were E.coli and Klebsiella pneumoniae and one was E.coli and Candida glabrata. In catheterized urine samples also the commonest cause of UTI was E.coli 42.02 % (n=29), closely followed by Klebsiella pneumoniae 37.68% (n=26). All the Candida species isolated are 100% sensitive to the commonly used antifungals like Amphotericin B, Fluconazole and Itraconazole. In present study, majority of patients recovered well (90 %) while 9 patients had complications. Conclusion: UTI is a common finding in patients with symptomatic BPH in our setting (44.33%). There is a steady increase in the prevalence of UTI with increasing age group, among BPH patient in this study.