Carbon monoxide is generated when fuels such as charcoal, fuel gas, briquette and petroleum undergo incomplete combustion. This can occur when using appliances like burner, cooking equipment or heater in poorly ventilated area as well as due to faulty water heaters, faulty water heaters, vehicles exhaust emissions, industrial activities and cigarette smoke exposure, resulting in elevated Carboxyhemoglobin levels. The impact of minimal exposure to carbon monoxide on an individual’s cognitive functions is not extensively documented as the clinical syndrome in hidden cases presents with a vague clinical picture and limited awareness and understanding in this particular field. A wide range of neurological may occur due to exposure to carbon monoxide including headache, dizziness, fatigue, lethargy, syncope, coma, seizures and potentially fatality at elevated levels. Research has shown that deep breathing exercises can assist in lowering the carboxyhemoglobin levels in indivisuals who smoke. The practice of Pranayama which involves regulating the breath, is a vital element of Yoga tradition. We carried out this research to examine association between Carboxyhemoglobin and Methhemoglobin levels with Yogic Breathing in healthy undergraduate medical students. This research comprises an analytical observational cross sectional study. Study was conducted at Rama Medical College, Hospital & Research Centre and Government Medical College, Jalaun (Orai) those are tertiary medical teaching institute of Uttar Pradesh State of India. Participating in the study were healthy undergraduate medical students. Study Period was scheduled for January 2023. The research was conducted on a group of 100 undergraduate medical students from two different medical institutions. The oxygen saturation level were found to be within normal range (>98%). We observed a notable difference in the Pulse rate (p<0.05) and carboxyhemoglobin level (p<0.02) across both groups. Practicing Yogic breathing is a beneficial approach to breathing, and therefore promoting the adoption of such healthy habits globally is highly encouraged.