Background: Thyroid function tests(TFT) and lipid profiles are crucial diagnostic tools for understanding body processes, assessing thyroid gland performance, and identifying primary thyroid disorders like hypothyroidism.Aim: The aim of the present study was to determine thyroid function test and lipid profilemarkers in hypothyroidism patients.Materials & methods: The study, conducted in the Department of Biochemistry, involved 300 participants aged 30-60 years, divided into two groups: 150 healthy individuals and 150 hypothyroidism patients. The study aimed to identify clinically diagnosed hypothyroid patients aged 30-60 years who have been on medication within the last five years. Patients with established conditions, chronic illnesses, pregnancy, recent surgical interventions, or those under 30 or over 60 years were excluded from the study.Results: T3, T4, fT3, fT4, and TSH levels are shown in Figure 1. All the thyroid profile tests were significant differed when compared between control group subjects and hypothyroid group patients. Significant differences were observed in the serum levels of lipid variables, apolipoproyein-A1 (Apo-a1), lipoprotein-A1 (lLpo-a1), and apolipoprotein-B (Apo-B) when hypothyroid group patients compared to control subjects.Conclusion:Thyroid dysfunction, including hypothyroidism, significantly impacts cardiovascular health and lipid profile markers, requiring early detection and management to reduce cardiac risk.