Background: Biofilm structures are complex entities that develop at wound surfaces. Microbes make an extracellular polymeric substance, or EPS, matrix. Biofilms are most commonly associated with chronic wounds, which are predisposed to delayed healing. Various topical agents, including honey and colloidal silver, have been used to eradicate biofilm, although the effectiveness of these treatments has been questioned. This was aimed at evaluating the relative effectiveness of honey and silver dressings regarding the eradication of biofilm, the process of wound healing, and general clinical outcomes.Methods: This is a prospective comparative study whereby, 168 patients were assessed for the biofilm and 72 patients that were positive were alternately assigned either to honey groups (n = 36) or silver dressing groups (n = 36) with the assessment of the wound done biweekly intervals of five days for biofilm reduction, wound discharge, foul smell, and granulation and length of hospital stay and time required for definitive treatment. Results: These results showed that the reduction in biofilm, wound discharge, and foul smell were alike for both honey and silver dressings. The purulent discharge in both groups had become serous by day 3. Formation of granulation tissue was documented in both groups, although this was a little quicker in the silver group. The average hospital stay was shorter in the honey group, 25.8±14.8 days versus 28.3±19.2 days in the silver group (p=0.54). Not surprisingly, time to definitive therapy was also a bit shorter in the honey group: 20.2±12.5 days versus 26.5±17.4 days (p=0.18). Conclusion: At the end, honey and silver dressings were found to be equal in their effectiveness toward healing varied chronic wounds. Both types show comparability in aspects such as reduction of biofilm, control exudate, and healing of the wound. Probably, the choice is going to be between honey and silver will depend on wound-specific characteristics, cost considerations, and patient preference. Further research with a larger sample size is much needed to provide more definitive guidelines for selection of local applicants.